Menu

BIO-DETAIL CORPORATION

Expertise in Adeno Associate Virus Packaging

AAV introduction

AAV is a small linear single-stranded DNA parvovirus with a diameter of 20 nm, a molecular weight of 3.9 MegaDaltons, and 60 monomers of VP1, VP2, and VP3 in a ratio of 1:1:10. It is replication-deficient and has traditionally required co-infection with a helper adenovirus or herpes virus for replication and infection.  Its advantages include being non-pathogenic and low immunogenic to humans. It also has high transduction efficiency and long-term transgenic expression and broad host range including both dividing and none-dividing cells. These advantages make AAV a most promising tool for gene therapy and biomedical research.

AAV genome

AAV genome is about 4.7 kilobases long comprised of inverted terminal repeats (ITRs), rep and cap.  Rep proteins are required for AAV genome replication and Cap proteins are composed of virus capsid.

AAV packaging

We are currently using PEI to co-transfect pAAV, pAAV-RC, and pHelper into HEK 293A cells to package AAV. We are conducting iodixanol gradient ultracentrifugation or precipitate and PEG / aqueous two-phase partitioning to produce higher titer and purity AAV vectors. We also provide heparin affinity purification upon customer request.

Promotions

Free trial AAV packaging service

Free trial AAV packaging service of the titer 5X10^11 vg available for the new customers. Please email to ordering@bio-detail.com for detail information.

Receive 8% off for the orders of 6 or more AAV packaging services

Receive 8% off for the orders of 6 or more AAV packaging services. Please send us an email at ordering@bio-detail.com for a quote.

Free samples for first 20 orders start today (expired)

First 20 customers who order AAV packaging service receive free 100 µl 1x10^13/ml control hrGFP or mCherry tagged control AAV virus (AAV2 only). Please comment with your order number or quote number below.

(This offer is expired, please contact us at ordering@bio-detail.com for a free sample re…

Read more